Journal article
S. Lee, Ravi Gupta, R. Jayaramaiah, S. H. Lee, Yiming Wang, Sangryeol Park, S. Kim
The plant pathology journal, 2017
The plant pathology journal, 2017
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DOI
PubMedCentral
PubMed
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APA
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Lee, S., Gupta, R., Jayaramaiah, R., Lee, S. H., Wang, Y., Park, S., & Kim, S. (2017). Global Transcriptome Profiling of Xanthomonas oryzae pv. oryzae under in planta Growth and in vitro Culture Conditions. The Plant Pathology Journal.
Chicago/Turabian
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Lee, S., Ravi Gupta, R. Jayaramaiah, S. H. Lee, Yiming Wang, Sangryeol Park, and S. Kim. “Global Transcriptome Profiling of Xanthomonas Oryzae Pv. Oryzae under in Planta Growth and in Vitro Culture Conditions.” The plant pathology journal (2017).
MLA
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Lee, S., et al. “Global Transcriptome Profiling of Xanthomonas Oryzae Pv. Oryzae under in Planta Growth and in Vitro Culture Conditions.” The Plant Pathology Journal, 2017.
BibTeX Click to copy
@article{s2017a,
title = {Global Transcriptome Profiling of Xanthomonas oryzae pv. oryzae under in planta Growth and in vitro Culture Conditions},
year = {2017},
journal = {The plant pathology journal},
author = {Lee, S. and Gupta, Ravi and Jayaramaiah, R. and Lee, S. H. and Wang, Yiming and Park, Sangryeol and Kim, S.}
}
Abstract
Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.